[HTML][HTML] Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling
Human pluripotent stem cells (hPSCs) offer the potential to generate large numbers of
functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that
temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac
induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-
catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte
specification, whereas glycogen synthase kinase 3 inhibition at this point enhanced …
functional cardiomyocytes from clonal and patient-specific cell sources. Here we show that
temporal modulation of Wnt signaling is both essential and sufficient for efficient cardiac
induction in hPSCs under defined, growth factor-free conditions. shRNA knockdown of β-
catenin during the initial stage of hPSC differentiation fully blocked cardiomyocyte
specification, whereas glycogen synthase kinase 3 inhibition at this point enhanced …