[HTML][HTML] Human retinal pigment epithelial cells prefer proline as a nutrient and transport metabolic intermediates to the retinal side
JR Chao, K Knight, AL Engel, C Jankowski… - Journal of Biological …, 2017 - ASBMB
Metabolite transport is a major function of the retinal pigment epithelium (RPE) to support the
neural retina. RPE dysfunction plays a significant role in retinal degenerative diseases. We
have used mass spectrometry with 13 C tracers to systematically study nutrient consumption
and metabolite transport in cultured human fetal RPE. LC/MS-MS detected 120 metabolites
in the medium from either the apical or basal side. Surprisingly, more proline is consumed
than any other nutrient, including glucose, taurine, lipids, vitamins, or other amino acids …
neural retina. RPE dysfunction plays a significant role in retinal degenerative diseases. We
have used mass spectrometry with 13 C tracers to systematically study nutrient consumption
and metabolite transport in cultured human fetal RPE. LC/MS-MS detected 120 metabolites
in the medium from either the apical or basal side. Surprisingly, more proline is consumed
than any other nutrient, including glucose, taurine, lipids, vitamins, or other amino acids …