Phorbol ester markedly increases the sensitivity of intestinal epithelial cells to 1, 25-dihydroxyvitamin D3
HJ Armbrecht, TL Hodam, MA Boltz, ML Chen - FEBS letters, 1993 - Elsevier
HJ Armbrecht, TL Hodam, MA Boltz, ML Chen
FEBS letters, 1993•ElsevierWe have used a clonal intestinal epithelial cell line (IEC-18) to study the mechanism of
action of 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D) in vitro. 1, 25 (OH) 2 D (10− 7 M)
elevated by over 10-fold the mRNA levels for the cytochrome P450 component (P450cc24)
of the 1, 25 (OH) 2 D-24-hydroxylase. Increased P450cc24 mRNA levels were detectable at
6 h and peaked at 36 h. Below a concentration of 10− 7 M, 1, 25 (OH) 2 D had almost no
effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1, 25 (OH) …
action of 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D) in vitro. 1, 25 (OH) 2 D (10− 7 M)
elevated by over 10-fold the mRNA levels for the cytochrome P450 component (P450cc24)
of the 1, 25 (OH) 2 D-24-hydroxylase. Increased P450cc24 mRNA levels were detectable at
6 h and peaked at 36 h. Below a concentration of 10− 7 M, 1, 25 (OH) 2 D had almost no
effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1, 25 (OH) …
Abstract
We have used a clonal intestinal epithelial cell line (IEC-18) to study the mechanism of action of 1,25-dihydroxyvitamin D3 (1,25(OH)2D) in vitro. 1,25(OH)2D (10−7 M) elevated by over 10-fold the mRNA levels for the cytochrome P450 component (P450cc24) of the 1,25(OH)2D-24-hydroxylase. Increased P450cc24 mRNA levels were detectable at 6 h and peaked at 36 h. Below a concentration of 10−7 M, 1,25(OH)2D had almost no effect. However, addition of phorbol ester for 2 h made the intestine responsive to 1,25(OH)2D concentrations as low as 10−9 M.
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