Systematic design and testing of nested (RT-) PCR primers for specific amplification of mouse rearranged/expressed immunoglobulin variable region genes from …
S Rohatgi, P Ganju, D Sehgal - Journal of immunological methods, 2008 - Elsevier
The aim of this study was to develop a highly specific and sensitive (RT-) PCR capable of
potentially amplifying the rearranged/expressed VH and VL gene belonging to any mouse
immunoglobulin V gene family from a single or a small number of B cells. A database of
germline immunoglobulin sequences was used to design 112 primers for a nested (RT-)
PCR based strategy to cover all VH, VL, JH, JL, CH and CL gene families/genes from
C57BL/6 and BALB/c mice. 93.7% of the primers had 4-fold or less, while 71.4% had no …
potentially amplifying the rearranged/expressed VH and VL gene belonging to any mouse
immunoglobulin V gene family from a single or a small number of B cells. A database of
germline immunoglobulin sequences was used to design 112 primers for a nested (RT-)
PCR based strategy to cover all VH, VL, JH, JL, CH and CL gene families/genes from
C57BL/6 and BALB/c mice. 93.7% of the primers had 4-fold or less, while 71.4% had no …