β-Glucan enhances cytotoxic T lymphocyte responses by activation of human monocyte-derived dendritic cells via the PI3K/AKT pathway
J Ding, T Feng, Y Ning, W Li, Q Wu, K Qian, Y Wang… - Human Immunology, 2015 - Elsevier
Purpose To investigate the effects of β-(1, 3/1, 6)-d-glucan on dendritic cells (DCs)
maturation, cytotoxic T lymphocyte responses and the molecular mechanisms of its
transition. Methods and results Human monocyte-derived DCs were matured using yeast-
derived particulate β-glucan (WGP) or a mix of TNF-α, IL-1β and IL-6 (“Conv mix”). Multicolor
flow cytometry was used to study the DCs phenotype and cytotoxic T-lymphocyte priming
and differentiation. ELISA and RT-PCR assays were used to evaluate cytokine production …
maturation, cytotoxic T lymphocyte responses and the molecular mechanisms of its
transition. Methods and results Human monocyte-derived DCs were matured using yeast-
derived particulate β-glucan (WGP) or a mix of TNF-α, IL-1β and IL-6 (“Conv mix”). Multicolor
flow cytometry was used to study the DCs phenotype and cytotoxic T-lymphocyte priming
and differentiation. ELISA and RT-PCR assays were used to evaluate cytokine production …