Antigen specific in vivo killing assay using CFSE labeled target cells
M Durward, J Harms, G Splitter - JoVE (Journal of Visualized Experiments), 2010 - jove.com
Carboxyfluorescein diacetate succinimidyl ester (CFSE) can be used to easily and quickly
label a cell population of interest for in vivo investigation. This labeling has classically been
used to study proliferation and migration. In the method presented here, we have shortened
the timeline after adoptive transfer to look at survival and killing of epitope specific CFSE
labeled target cells4-6. The level of specific killing of a CD8+ T cell clone can indicate the
quality of the response, as their quantity may be misleading. Specific CD8+ T cells can …
label a cell population of interest for in vivo investigation. This labeling has classically been
used to study proliferation and migration. In the method presented here, we have shortened
the timeline after adoptive transfer to look at survival and killing of epitope specific CFSE
labeled target cells4-6. The level of specific killing of a CD8+ T cell clone can indicate the
quality of the response, as their quantity may be misleading. Specific CD8+ T cells can …