About the possible origin and mechanism of circulating DNA: Apoptosis and active DNA release
M Stroun, J Lyautey, C Lederrey, A Olson-Sand… - Clinica chimica acta, 2001 - Elsevier
M Stroun, J Lyautey, C Lederrey, A Olson-Sand, P Anker
Clinica chimica acta, 2001•ElsevierBackground: In addition to cell lysis, apoptosis has been advanced as the origin of
circulating DNA on the basis of several observations. Plasma or serum DNA often presents a
ladder pattern reminiscent of that displayed by apoptotic cells when subjected to
electrophoresis. However, the phenomenon of active release of DNA from cells might also
be expected to result in a ladder pattern on electrophoresis. Non-dividing cells, such as
lymphocytes, frog auricles and cultured cell lines including HL-60, spontaneously release a …
circulating DNA on the basis of several observations. Plasma or serum DNA often presents a
ladder pattern reminiscent of that displayed by apoptotic cells when subjected to
electrophoresis. However, the phenomenon of active release of DNA from cells might also
be expected to result in a ladder pattern on electrophoresis. Non-dividing cells, such as
lymphocytes, frog auricles and cultured cell lines including HL-60, spontaneously release a …
Background: In addition to cell lysis, apoptosis has been advanced as the origin of circulating DNA on the basis of several observations. Plasma or serum DNA often presents a ladder pattern reminiscent of that displayed by apoptotic cells when subjected to electrophoresis. However, the phenomenon of active release of DNA from cells might also be expected to result in a ladder pattern on electrophoresis. Non-dividing cells, such as lymphocytes, frog auricles and cultured cell lines including HL-60, spontaneously release a nucleoprotein complex within a homeostatic system in which newly synthesized DNA is preferentially released. Conclusion: In relation to DNA synthesis, the phenomenon of extracellular DNA in different culture conditions favors apoptosis or spontaneous active DNA release.
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