Regulation of SRp20 exon 4 splicing
H Jumaa, PJ Nielsen - Biochimica et Biophysica Acta (BBA)-Gene Structure …, 2000 - Elsevier
SR proteins are essential splicing factors involved in the use of both constitutive and
alternative exons. We previously showed that the SR proteins SRp20 and ASF/SF2 have
antagonistic activities on SRp20 pre-mRNA splicing. SRp20 activates exon 4 recognition in
its pre-mRNA, whereas ASF/SF2 inhibits this recognition. In experiments aimed at testing the
specificity of SRp20 and ASF/SF2 for exon 4 splicing regulation, we show here that this
specificity lies in the RNA binding domains of SRp20 and ASF/SF2 and not in the RS …
alternative exons. We previously showed that the SR proteins SRp20 and ASF/SF2 have
antagonistic activities on SRp20 pre-mRNA splicing. SRp20 activates exon 4 recognition in
its pre-mRNA, whereas ASF/SF2 inhibits this recognition. In experiments aimed at testing the
specificity of SRp20 and ASF/SF2 for exon 4 splicing regulation, we show here that this
specificity lies in the RNA binding domains of SRp20 and ASF/SF2 and not in the RS …