LPS Tolerance in Monocytes/Macrophages: Three 3'Cytosins are Required in the DNA Binding Motif for Detection of Upregulated NF-κB p50 Homodimers
M Frankenberger, HWL Ziegler-Heitbrock - Immunobiology, 1997 - Elsevier
When monocytes are stimulated with LPS (lipopolysaccharide) repeatedly then the initially
high expression of the TNF (tumor necrosis factor) gene is only very low, ie the cells are
tolerant to LPS. Tolerant cells still express the CD14 receptor and they can still be activated
to mobilize NF-κB into nucleus. Analysis of the binding proteins employing the-605 motif of
the human TNF promoter (GGGGCTGTCCC) revealed that in tolerant cells of the human
monocytic cell line Mono Mac 6 there is a predominance of p50p50 of NF-κB. We now show …
high expression of the TNF (tumor necrosis factor) gene is only very low, ie the cells are
tolerant to LPS. Tolerant cells still express the CD14 receptor and they can still be activated
to mobilize NF-κB into nucleus. Analysis of the binding proteins employing the-605 motif of
the human TNF promoter (GGGGCTGTCCC) revealed that in tolerant cells of the human
monocytic cell line Mono Mac 6 there is a predominance of p50p50 of NF-κB. We now show …