[HTML][HTML] Detection of reactive oxygen species via endogenous oxidative pentose phosphate cycle activity in response to oxygen concentration: implications for the …
SW Tuttle, A Maity, PR Oprysko, AV Kachur… - Journal of biological …, 2007 - ASBMB
The oxidative pentose phosphate cycle (OPPC) is necessary to maintain cellular reducing
capacity during periods of increased oxidative stress. Metabolic flux through the OPPC
increases stoichiometrically in response to a broad range of chemical oxidants, including
those that generate reactive oxygen species (ROS). Here we show that OPPC sensitivity is
sufficient to detect low levels of ROS produced metabolically as a function of the percentage
of O 2. We observe a significant decrease in OPPC activity in cells incubated under severe …
capacity during periods of increased oxidative stress. Metabolic flux through the OPPC
increases stoichiometrically in response to a broad range of chemical oxidants, including
those that generate reactive oxygen species (ROS). Here we show that OPPC sensitivity is
sufficient to detect low levels of ROS produced metabolically as a function of the percentage
of O 2. We observe a significant decrease in OPPC activity in cells incubated under severe …