[HTML][HTML] Human placenta thioredoxin reductase: Isolation of the selenoenzyme, steady state kinetics, and inhibition by therapeutic gold compounds
S Gromer, LD Arscott, CH Williams, RH Schirmer… - Journal of Biological …, 1998 - ASBMB
Human thioredoxin reductase is a pyridine nucleotide-disulfide oxidoreductase closely
related to glutathione reductase but differing from the latter in having a Cys-SeCys
(selenocysteine) sequence as an additional redox center. Because selenoproteins cannot
be expressed yet in heterologous systems, we optimized the purification of the protein from
placenta with respect to final yield (1–2 mg from one placenta), specific activity (42 units/mg),
and selenium content (0.94±0.03 mol/mol subunit). The steady state kinetics showed that the …
related to glutathione reductase but differing from the latter in having a Cys-SeCys
(selenocysteine) sequence as an additional redox center. Because selenoproteins cannot
be expressed yet in heterologous systems, we optimized the purification of the protein from
placenta with respect to final yield (1–2 mg from one placenta), specific activity (42 units/mg),
and selenium content (0.94±0.03 mol/mol subunit). The steady state kinetics showed that the …