Publisher Summary Plasma lipoproteins have lower hydrated densities relative to the other
plasma proteins; therefore sequential flotation ultracentrifugation has been the principal
method used for their isolation and classification. This chapter describes the sequential
flotation ultracentrifugation method and some important features for the proper use of
sequential flotation ultracentrifugation, such as it is important to calculate the effect of
differences in path length or it is virtually impossible to reduce the level of contaminating …