Human CtIP mediates cell cycle control of DNA end resection and double strand break repair
P Huertas, SP Jackson - Journal of Biological Chemistry, 2009 - ASBMB
In G 0 and G 1, DNA double strand breaks are repaired by nonhomologous end joining,
whereas in S and G 2, they are also repaired by homologous recombination. The human
CtIP protein controls double strand break (DSB) resection, an event that occurs effectively
only in S/G 2 and that promotes homologous recombination but not non-homologous end
joining. Here, we mutate a highly conserved cyclin-dependent kinase (CDK) target motif in
CtIP and reveal that mutating Thr-847 to Ala impairs resection, whereas mutating it to Glu to …
whereas in S and G 2, they are also repaired by homologous recombination. The human
CtIP protein controls double strand break (DSB) resection, an event that occurs effectively
only in S/G 2 and that promotes homologous recombination but not non-homologous end
joining. Here, we mutate a highly conserved cyclin-dependent kinase (CDK) target motif in
CtIP and reveal that mutating Thr-847 to Ala impairs resection, whereas mutating it to Glu to …
