Association between HaeIII polymorphism of scavenger receptor class B type I gene and plasma HDL-cholesterol concentration
SH Hong, YR Kim, YM Yoon, WK Min… - Annals of clinical …, 2002 - journals.sagepub.com
SH Hong, YR Kim, YM Yoon, WK Min, SI Chun, JQ Kim
Annals of clinical biochemistry, 2002•journals.sagepub.comBackground: Evidence has recently been found for significant associations between genetic
variation within the scavenger receptor class B type I gene (SR-BI), plasma lipids and
anthropometric measurements in healthy Caucasians. The present case-control study was
conducted to determine whether there is an association between three polymorphisms
identified by the restriction endonucleases Hae III, Alu I and Apa I of SR-BI and coronary
artery disease (CAD) in Korean subjects. Methods: DNA was extracted from 137 subjects …
variation within the scavenger receptor class B type I gene (SR-BI), plasma lipids and
anthropometric measurements in healthy Caucasians. The present case-control study was
conducted to determine whether there is an association between three polymorphisms
identified by the restriction endonucleases Hae III, Alu I and Apa I of SR-BI and coronary
artery disease (CAD) in Korean subjects. Methods: DNA was extracted from 137 subjects …
Background: Evidence has recently been found for significant associations between genetic variation within the scavenger receptor class B type I gene (SR-BI), plasma lipids and anthropometric measurements in healthy Caucasians. The present case-control study was conducted to determine whether there is an association between three polymorphisms identified by the restriction endonucleases HaeIII, AluI and ApaI of SR-BI and coronary artery disease (CAD) in Korean subjects.
Methods: DNA was extracted from 137 subjects with CAD and 124 age-matched controls; it was amplified using the polymerase chain reaction. Individual alleles at each of the three polymorphic sites were identified by digestion with the appropriate restriction enzyme.
Results: Only a single allele was identified at the AluI and ApaI polymorphic sites. The frequency of the common (+) allele at the HaeIII polymorphic site was higher in CAD patients than in the controls (P = 0·001). The concentrations of plasma HDL-cholesterol and apolipoprotein AI also varied significantly among HaeIII genotypes in the CAD patients. The common (+) allele of the HaeIII polymorphism was associated with a lower body mass index in female controls.
Conclusions: Allele frequencies of the AluI and ApaI polymorphisms in this study were different to those in a Caucasian population studied previously, suggesting a difference in the genetic background. Further comparative studies of SR-BI polymorphism in other racial or ethnic groups should therefore prove to be of value.
Sage Journals