Pretreatment of plastic Petri dishes with fetal calf serum. A simple method for macrophage isolation
K Kumagai, K Itoh, S Hinuma, M Tada - Journal of immunological methods, 1979 - Elsevier
We have developed a simple method which can recover the highly purified macrophages or
monocytes in suspension from mouse peritoneal exudate cells and human peripheral blood
mononuclear cells. Plastic Petri dishes coated overnight with heat-inactivated fetal calf
serum (FCS) selectively bind macrophages and monocytes. The adherent macrophages
and monocytes are easily removed by incubation in phosphate-buffered saline containing
0.2% ethylenediamine tetraacetate (EDTA) and 5% FCS, and recovered as a cell …
monocytes in suspension from mouse peritoneal exudate cells and human peripheral blood
mononuclear cells. Plastic Petri dishes coated overnight with heat-inactivated fetal calf
serum (FCS) selectively bind macrophages and monocytes. The adherent macrophages
and monocytes are easily removed by incubation in phosphate-buffered saline containing
0.2% ethylenediamine tetraacetate (EDTA) and 5% FCS, and recovered as a cell …