Animal models of fungal and other infectious diseases often require that the number of
organisms in tissue be quantified, traditionally by grinding organs, plating them on agar and
counting colony forming units (CFU). This method is labor intensive, slow as some fungi
require two weeks of culture and limited in reliability by poor plating efficiency. To circumvent
these problems, we developed a flow cytometric method to quantify yeast. In vitro cultured
Blastomyces dermatitidis, Cryptococcus neoformans, Candida albicans and Histoplasma …