[HTML][HTML] Cloning of a cDNA for the type II iodothyronine deiodinase
JC Davey, KB Becker, MJ Schneider… - Journal of Biological …, 1995 - ASBMB
Three types of iodothyronine deiodinase have been identified in vertebrate tissues. cDNAs
for the types I and III have been cloned and shown to contain an in-frame TGA that codes for
selenocysteine at the active site of the enzyme. We now report the cloning of a cDNA for a
type II deiodinase using a reverse transcription/polymerase chain reaction strategy and RNA
obtained from Rana catesbeiana tissues. This cDNA (RC5′ DII) manifests limited but
significant homology with other deiodinase cDNAs and contains a conserved in-frame TGA …
for the types I and III have been cloned and shown to contain an in-frame TGA that codes for
selenocysteine at the active site of the enzyme. We now report the cloning of a cDNA for a
type II deiodinase using a reverse transcription/polymerase chain reaction strategy and RNA
obtained from Rana catesbeiana tissues. This cDNA (RC5′ DII) manifests limited but
significant homology with other deiodinase cDNAs and contains a conserved in-frame TGA …
