A bioactive 60-kilodalton prolactin species is preferentially secreted in cultures of mitogen-stimulated and nonstimulated peripheral blood mononuclear cells from …
F Larrea, A Martínez-Castillo, V Cabrera… - The Journal of …, 1997 - academic.oup.com
F Larrea, A Martínez-Castillo, V Cabrera, J Alcocer-Varela, G Queipo, C Cariño…
The Journal of Clinical Endocrinology & Metabolism, 1997•academic.oup.comWe have evaluated the production of PRL by human peripheral mononuclear cells (PBMNC)
from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned
medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence
of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In
the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P< 0.001)
amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned …
from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned
medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence
of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In
the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P< 0.001)
amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned …
We have evaluated the production of PRL by human peripheral mononuclear cells (PBMNC) from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P < 0.001) amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned medium was inhibited with specific antiserum to human PRL. Western blotting using a polyclonal antibody to human PRL revealed a single 60-kDa PRL-like species in both normal and SLE PBMNC extracts, the immunoreactivity of which was preferentially found in SLE subjects. With the use of reverse transcription-PCR an expected 633-bp band was observed, and its similarity to pituitary PRL was further confirmed by Southern blot analysis with human PRL complementary DNA as a probe. We conclude that a high molecular mass PRL-like species is synthesized and secreted by PBMNC, and patients with SLE have an increased secretion of lymphocyte-derived PRL-like material.
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