An improved diphenylamine method for the estimation of deoxyribonucleic acid

KW Giles, A Myers - Nature, 1965 - nature.com
KW Giles, A Myers
Nature, 1965nature.com
Many plant tissues contain very low levels of DNA and in such circumstances it is necessary
to utilize a method of estimation of extreme sensitivity coupled with a low reagent blank. In
this respect it is our experience that the Burton diphenylamine method1 is the most
satisfactory. This procedure has been simplified, the blank considerably reduced and the
sensitivity increased.
Abstract
Many plant tissues contain very low levels of DNA and in such circumstances it is necessary to utilize a method of estimation of extreme sensitivity coupled with a low reagent blank. In this respect it is our experience that the Burton diphenylamine method1 is the most satisfactory. This procedure has been simplified, the blank considerably reduced and the sensitivity increased.
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