Identification of a novel protein kinase A anchoring protein that binds both type I and type II regulatory subunits
Compartmentalization of cAMP-dependent protein kinase is achieved in part by interaction
with A-kinase anchoring proteins (AKAPs). All of the anchoring proteins identified previously
target the kinase by tethering the type II regulatory subunit. Here we report the cloning and
characterization of a novel anchoring protein, D-AKAP1, that interacts with the N terminus of
both type I and type II regulatory subunits. A novel cDNA encoding a 125-amino acid
fragment of D-AKAP1 was isolated from a two-hybrid screen and shown to interact …
with A-kinase anchoring proteins (AKAPs). All of the anchoring proteins identified previously
target the kinase by tethering the type II regulatory subunit. Here we report the cloning and
characterization of a novel anchoring protein, D-AKAP1, that interacts with the N terminus of
both type I and type II regulatory subunits. A novel cDNA encoding a 125-amino acid
fragment of D-AKAP1 was isolated from a two-hybrid screen and shown to interact …
